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AAT Bioquest

FAQ

Posted on August 7, 2023
What are the advantages of allophycocyanin (APC)?
Antibodies and ProteomicsAntibody and Protein LabelingCellular PorcessesFlow Cytometry ReagentsPE and APC


Posted on August 7, 2023
What guidelines should I follow for successful tandem dye usage?
Chemical Reagents Förster Resonance Energy Transfer (FRET)Antibody and Protein LabelingFlow Cytometry ReagentsPE and APC


Posted on October 20, 2021
What are the 3 top guidelines for using PerCP conjugates and tandems?
PE and APCBioconjugation


Posted on September 27, 2021
What does a conjugated antibody mean?
Horseradish Peroxidase (HRP) and Poly-HRPPE and APCSecondary AntibodiesAntibodies and Proteomics


Posted on September 24, 2021
What is pre-adsorption?
Secondary AntibodiesPE and APCBioconjugationProtein to Protein Conjugation


Posted on September 24, 2021
What steps can be taken to avoid degradation of tandem dyes?
PE and APCBioconjugation


Posted on September 24, 2021
What is the major limitation of tandem dyes?
PE and APCBioconjugation


Posted on September 23, 2021
Are tandem dyes sensitive to fixatives and permeabilization agents?
PE and APCBioconjugation


Posted on September 23, 2021
Why are tandem dyes not recommended for intracellular staining?
BioconjugationPE and APC


Posted on September 23, 2021
What are tandem dyes? What is the advantage of using tandem dyes?
BioconjugationProtein to Protein ConjugationPE and APC


Posted on July 1, 2021
Is PE Cy7 photosensitive?
PE and APCClassic DyesBioconjugation


Posted on July 1, 2021
Can cyanine dyes be used in tandem with other dyes?
PE and APCCyaninesClassic Dyes


Posted on June 22, 2020
Why spectral flow cytometry is advantageous over conventional flow cytometry in multi-color experiments?
Flow Cytometry ReagentsFluorescencePE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is the advantage of tandem dyes in flow cytometry?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What are tandem dyes in flow cytometry?
Bioconjugation Förster Resonance Energy Transfer (FRET)CyaninesFRETFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on May 9, 2020
Is there a spectral overlap between PerCP-Cy5.5 and PE-Cy5?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsImmunohistochemistry (IHC)PE and APCSpectral Flow Cytometry


Posted on May 9, 2020
What is the number of events in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCPhysiological ProbesSpectral Flow Cytometryflow cytometryiFluor Dyes and Kitsmultiplex